![]() An increasing interest in non-surgical methods of oocyte recovery, as well as recovery and transfer of embryos for research purposes and practical implementation can be seen. The method allows for a fast and effective conduct of the operation in a living donor with minimal invasiveness while preserving the excellent condition of animals.Įndoscopic methods are used in breeding animals for recovering and transferring embryos, for intra-uterine insemination, and for diagnostic purposes, e.g., ovary and uterus evaluation, ovulation observation and development of corpus luteum, and oocyte recovery. ![]() The results of the study show that the proposed technique of laparoscopic recovery of oocytes allows a sufficient number of ovarian cells suitable for in vitro culture to be obtained and as a consequence it makes them useful in in vitro maturation/in vitro fertilization (IVM/IVF) programs or cloning. After evaluation, 94% (both Variant I and II), 93% (Variant III), and 84% (Variant IV) of the oocytes were qualified for in vitro maturation. Recovery rates were 35% (Variant I), 57% (Variant II), 72% (Variant III), and 67% (Variant IV). Various techniques of recovering oocytes were discussed, including the techniques of conducting the operation, various tools for recovering oocytes, and different plans of hormonal stimulation. ![]() In this study, four different L-OPU variants were described in goats and sheep. Therefore, it is necessary to develop effective non-invasive techniques allowing for multiple good quality oocyte recovery that would be suitable for in vitro maturation and fertilization. ![]() The limitation of using the laparoscopic ovum pick-up (L-OPU) method in goat and sheep is its changing effectiveness and the lack of repeatability of results, as well as the varying effectiveness of different variants of the method. It allows for good quality oocytes that are suitable for in vitro maturation and fertilization to be recovered. ![]() The laparoscopic method of recovering oocytes in goats and sheep is one of the minimally invasive methods used in the biotechnology of animal reproduction. ![]()
0 Comments
Leave a Reply. |